Table 1.

Relative levels of Bcl6 mRNA and hnRNA in rat liver

Bcl6	qPCR primer location	Relative RNA levels (%)
		Male	Female
mRNA	Exon 3/exon 4	100 ± 18	5.4 ± 0.9
	Exon 9/exon 10	100 ± 17	7.2 ± 0.7
hnRNA	Exon 3/intron 3	100 ± 14	531 ± 44
	Exon 5/intron 5	19 ± 5	2.4 ± 0.3

Relative mRNA and hnRNA levels were determined by qPCR analysis of individual untreated adult male (n = 29) and female (n = 20) rat liver RNA samples (as in Fig. 1A) using primer pairs complementary to Bcl6 exon 3/exon 4 and exon 9/exon 10 sequences, for mRNA analysis, and Bcl6 exon 3/intron 3 and exon 5/intron 5 sequences for hnRNA. RNA levels were normalized to the 18S rRNA content of each liver. The levels of mRNA are presented relative to the average level of the corresponding mRNA in males, which was set to 100%. Bcl6 hnRNA levels are adjusted for the primer efficiency and calculated relative to the average male level of hnRNA containing exon 3/intron 3 junction, which was set to 100%. Data are mean values ± se. Bcl6 mRNA levels determined with the two sets of exonic primer pairs showed an excellent correlation (r² = 0.98) across the full set of 49 livers.