Figure 1. Canonical pathways for alternatively spliced and differentially expressed T cell genes (p<0.001) shows both immune/inflammatory mechanisms and metabolism.

(A) Venn diagram representing the alternatively spliced and differentially expressed (AS+DE) genes detected at 24, 48, and 72 hours in activated T cells, with total number of detected genes per time point shown in parenthesis, number of genes unique to each time point shown by the number inside the circle and number of genes shared between time points in the shaded overlapping areas, with number of genes detected at all three time points shown in the central overlap. (B–F) Pie charts representing the Ingenuity Pathway Analysis output for the top canonical pathways populated by AS+DE genes during T cell activation: (B) shared at all time points, (C) genes unique to 24 hour time-point, (D) genes unique to the 48 hour time-point, (E) genes unique to the 72 hour time-point, (F) genes uniquely detected during the transition between 24 and 48 hour time-points. Numbers next to pathway name indicate the number of detected genes that are assigned to each pathway. The size of each shaded slice is proportional to the number of genes per pathway compared to total number of genes per chart.