Table 1. Antibody Panel Used In This Study.
Antigen | Clone | Dilution | Antigen Retrieval | Vendor |
CRX | Polyclonal Rabbit (H-120) | 1∶100 | Citrate; Microwave | Santa Cruz Biotechnology, Santa Cruz, CA |
OCT3/4(POU5F1) | Monoclonal Mouse (C-10; sc-5279) | 1∶2000 | Citrate, Steamer | Santa Cruz Biotechnology, Santa Cruz, CA |
OLIG2 | Polyclonal Rabbit (AB9610) | 1∶15K | Citrate; pressure cooker | Millipore |
GFAP | Polyclonal Rabbit (Z 0334) | 1∶20K | Pressure cooker citrate | DAKO |
Synaptophysin | Monoclonal Mouse (SY38) | 1∶200 | no treatment | DAKO |