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Immunohistochemistry for insulin and MafA in the islets of 20-wk-old mice. Double immunostaining for insulin (green) and MafA (red). MafA was observed only in the nucleus of β-cells in normoglycemic db/db-GPx(+) mice, whereas it was only in the cytoplasm of β-cells in hyperglycemic db/db-GPx(−) mice (n = 3) (magnification, ×40).
