Table 1.

Primers sequences and PCR conditions

Genes	Sequences (5′–3′)	Size (bp)	Cycles	Temperature (°C)
CSF-1
Forward	catccaggcagagactgaca	381	30	55
Reverse	cttgctgatcctccttccag
CSF-1R
Forward	gacctgctccacttctccag	304	30	55
Reverse	ggttcagaccaagcgagaag
CTR
Forward	tgctatgaccggattcatca	383	30	55
Reverse	gtcaccctctggcagctaag
Nox1
Forward	acagaggagagcttgggtga	320	30	60
Reverse	cactccaggaaggaaatgga
Nox4
Forward	ccagaatgaggatcccagaa	329	30	60
Reverse	aaaaccctcgaggcaaagat
Osterix
Forward	cttaacccagctccctaccc	492	30	60
Reverse	gtggtcgcttctggtaaagc
Osteocalcin
Forward	gcgctctgtctctctgacct	106	30	60
Reverse	gccggagtctgttcactacc
Procollagen 1
Forward	acgtcctggtgaagttggtc	170	30	60
Reverse	cagggaagcctctttctcct
RANKL
Forward	agccgagactacggcaagta	495	30	55
Reverse	gatggtgaggtgtgcaaatg
OPG
Forward	accaaagtgaatgccgagag	442	30	55
Reverse	ttgtgaagctgtgcaggaac
18S
Forward	gaccataaacgatgccgact	389	20	60
Reverse	gaacgccacttgtccctcta