Figure 10.
Effect of dexamethasone on E2-induced changes in gelatinase activity. Gelatin zymography analysis is shown for MMP-2 and MMP-9 activity from animals pretreated with saline or dexamethasone (Dex: 600 μg/kg) 20 h and 4 h prior to administration of E2 (40 μg/kg) or saline given 4 h before tissue collection. Zymogram (top) is representative of 6 gels using 3 separate pools of total protein extracted from different animals. Quantitative data (bottom) represents analysis of band intensity using AlphaEase FC software (Alpha Innotech) as described in the Methods; one way ANOVA, values represent mean ± SE for 6 gels. *P < 0.05 compared to saline control (Sal-Sal).