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. 2009 Nov 4;7:124. doi: 10.1186/1477-7827-7-124

Figure 4.

Figure 4

Temporal changes in uterine MMP mRNA and protein in response to E2. Uterine mRNA analysis via TaqMan® real-time PCR (panel A) and protein analysis via Western blot (panel B) are shown for MMP-2, MMP-3, MMP-7 and MMP-9 from uteri of OVX rats 0-72 h after treatment with saline (0 h control) or E2 (40 μg/kg). Messenger RNA values (panel A) represent mean ± SE, n = 6 tissues per group; one way ANOVA, *P < 0.05 compared to 0 h time point. Western blot images (panel B) are representative of a minimum of 3 blots using 3 separate pools of total protein extracted from different animals. Intensities of protein bands were quantitated using AlphaEase FC software (Alpha Innotech) as described in the Methods and values for each time point expressed as percent of saline control (100%). Quantitative data (panel B) reflect average band intensity values ± SE measured from ≥ 3 blots with total protein extracted from different animals in 2 separate experiments; one way ANOVA, *P < 0.05 compared to 0 h time point. IDV = integrated density value. Western blot analysis of β-actin as a control for gel loading is shown for the protein samples used to assess MMP protein for the blots above.