Fig. 2.

Comparison of cell adhesion molecule expression on astrocytes derived from neurospheres or mixed glial cultures. Mixed glial cultures (MGC) and pure astrocyte cultures were prepared as described in the Materials and Methods section, switched to serum-free conditions for two days, and then analysed for cell surface expression of the integrin subunits α1, α5, and α6, and the β-dystroglycan subunit (isotype control antibody: shaded filled line; integrin or dystroglycan antibodies: open line). These expression profiles are representative of three different experiments performed. Note that pure astrocytes and mixed glial astrocytes expressed a very similar profile of integrin subunits and dystroglycan.