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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Virology. 2009 Sep 12;394(1):28–38. doi: 10.1016/j.virol.2009.08.003

Fig. 3.

Fig. 3

Cytosolic localization of p33 replication protein in ssa1ts yeast grown at 36°C. (A) Confocal laser microscopy shows the localization of YFP-p33 in ssa1ts yeast and SSA1wt yeast grown at either 23°C or 36°C for 14 hours after induction from GAL1 promoter. The punctate structures show peroxisomal localization, while the “doughnut” -shape structure indicates cytosolic localization of p33 in yeast cells. The punctate structures formed in ssa1ts yeast grown at 23°C are reminiscent of those found in SSA1wt yeast (left side of the panel). (B) Cytosolic localization of peroxisome-targeted GFP, termed GFP-SKL, in ssa1ts yeast grown at 36°C for 14 hours after induction from GAL1 promoter. Note that GFP-SKL carries the peroxisomal targeting sequence “SKL” at the C-terminus.