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Quantitative real-time PCR. RT-PCR for renal (A) 1α(OH)ase and (B) klotho mRNA of control (CTRL), Fgf -23−/− (KO), Fgf -23−/−/hFGF-23-Tg double-mutant (DM), and col 1-FGF-23-Tg (TG) littermates at 9 weeks of age (n = 3 for each genotype). **p < 0.01 and ****p < 0.0001, statistically significant difference compared with controls. ####p < 0.0001, statistically significant difference compared with Fgf -23−/−
