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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Dev Biol. 2009 Apr 17;331(1):14–25. doi: 10.1016/j.ydbio.2009.04.015

Fig. 5.

Fig. 5

hlh-2 is required for niche function. (A-D) Early adult hermaphrodites, expressing lag-2::GFP (qIs57). (A, B) Top panel, Nomarski image. Middle panel, fluorescence. Bottom panel, fluorescence with brightness and contrast adjustment to enhance visualization of hDTC processes. (A) Control RNAi. The hDTC brightly expresses lag-2::GFP and extends processes proximally. (B) hlh-2 RNAi. The hDTC lag-2::GFP expression is diminished in cell body and processes. (C, D) Adult hermaphrodite germ lines extruded at 24 h past L4, and stained with DAPI. Animals also expressed lag-2::GFP (qIs57). White, DAPI. Green, lag-2::GFP. Feeding RNAi treatment was started late in the L3 stage. White bar, length of mitotic region. (C) Control RNAi. Mitotic region (MR) length extends approximately 20 cell diameters. hDTC is positioned at the distal end and brightly expresses lag-2::GFP. (D) 5’ hlh-2 RNAi. MR length is greatly reduced. The hDTC is slightly proximally displaced and faintly expresses lag-2::GFP. (E) Quantification of MR length (in cell diameters). MR length is dramatically reduced in hlh-2 RNAi-treated hermaphrodites. Control RNAi: 20 ± 0.6 (n=38); 5’ hlh-2 RNAi: 6 ± 1 (n=36); 3’ hlh-2 RNAi: 6 ± 1 (n=42). (F) Quantification of MR cell number from germ lines with the mean MR length. The number of cells in the MR is dramatically reduced in hlh-2 RNAi-treated hermaphrodites. Control RNAi: 222 ± 25 (n=5); 5’ hlh-2 RNAi: 68 ± 15 (n=6); 3’ hlh-2 RNAi: 71 ± 17 (n=5).