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. 2009 Sep 30;29(39):12045–12058. doi: 10.1523/JNEUROSCI.1365-09.2009

Figure 4.

Figure 4.

NR2 ATD control of open probability evaluated by the degree of MTSEA potentiation. A, Representative two-electrode voltage-clamp current recordings obtained from oocytes coexpressing recombinant NR1-A652C with NR2A, NR2A-ΔATD, NR2A-(2D-ATD), NR2D, NR2D-ΔATD, or NR2D-(2A-ATD) to evaluate the degree of potentiation by 0.2 mm MTSEA after the receptors are activated by glutamate and glycine (100 μm). Application of MTSEA to agonist-bound NR1-A652C-containing channels irreversibly locks open the pore following covalent modification. MTSEA-modified receptors have an open probability (POPEN) of ∼1.0, which renders the degree of MTSEA potentiation of the maximal agonist response inversely related to the open probability of the channel. B–D, Summary of the degree of MTSEA potentiation. Open probability estimated from these data are given in Table 3; #p < 0.01, one-way ANOVA with Bonferroni's post hoc test, compared with the corresponding wild type. *p < 0.01, unpaired t test, compared with the corresponding wild type.