Figure 5.

ATD deletion from NR2A decreases the single-channel open probabilities in outside-out patch-clamp recordings from transiently transfected HEK 293 cells. Aa1, Bb1, Steady-state recordings of NR1/NR2A (Aa1) and NR1/NR2A-ΔATD (Bb1) unitary currents in an outside-out patch that contained one active channel activated by 50 μm glycine and 1 mm glutamate are shown at different time scales. Aa2 and Bb2 show the open period histogram, shut duration histogram, amplitude histogram, and the stability plot of the amplitude for the channel in this patch.