Table 5.

NR2D-ATD alters τ3 describing the shut duration histogram

Control	NR1/NR2A experiment	τ2 (ms) control	τ2 (ms) exp	Con/exp (%)	τ3 (ms) control	τ3 (ms) exp	Con/exp (%)	Reference
Glutamate	Homoquinolinate	0.49	0.46	110%	4.7	6.2	130%	Erreger et al., 2005b
pH 7.3	pH 6.7	0.79	0.71	90%	4.3	7.4	170%	Unpublisheda
EDTA	300 nm Zn2+	1	0.63	63%	4	8	200%	Erreger and Traynelis, 2008
NR2A	NR2A-(2D-ATD)	0.91	1.3	140%	2.8	13	460%	Current study

Two intraburst shut duration constants (excluding τ0, the ultrafast 0.02 ms shut duration resolved using time course fitting for NR2A/D chimera in this study, and τ1) are compared for recombinant NR1/NR2A receptor, evaluated in outside out patches in control and the indicated experimental conditions. All experiments were performed in outside out patches at pH 7.3 in response to 100 μm glutamate 30 μm glycine in the presence of 10 μm EDTA to chelate contaminant Zn²⁺, except where indicated and for NR2A-NR2D ATD chimera, pH 8.0. Paired recordings were made from the same patch in control and experimental conditions. Fitted time constants are given for control recordings and the indicated experimental manipulations, and the percent change is shown. Data for homoquniolinic acid and 300 nm Zn²⁺ were from Erreger et al. (2005b) and Erreger and Traynelis (2008) and are included for comparison to data from this report.

^aComposite shut duration histograms were constructed from recordings of NR1/NR2A in outside out patches (n = 7), and fitted as described in Materials and Methods (K. Erreger and S. F. Traynelis, unpublished data).