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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: J Immunol. 2009 Apr 1;182(7):4459–4470. doi: 10.4049/jimmunol.0801450

FIGURE 4.

FIGURE 4

HIV-1 binding via CCR5 induces resistance in in vitro MDM cultures. Constitutive apoptosis in MDMs is shown in a and f. MDM binding by R5 tropic virus mediates MDM apoptosis resistance in vitro. CdCl2 induces cultured monocyte apoptosis (b) that is decreased upon preincubation with infectious HIV-1 BAL (c). Results represent the median ± SD for percentage of caspase-3 (d) and percentage of live (e) cells of three donors that were untreated (C), treated with CdCl2, and preincubated with HIV-1 BAL (HIV-1 BAL ± CdCl2). CdCl2 induces cultured monocyte apoptosis (g) that is decreased upon preincubation with noninfectious AT2R5 (h). CCR5 binding inhibitor M657 reverses AT2R5-mediated protection (i). Results represent the median ± SD for percentage of live (j) cells in 5 donors that were untreated (C), treated with CdCl2, preincubated with M657 (Inh + CdCl2), preincubated with AT2R5 (AT2R5 + CdCl2), and preincubated with M657 and AT2R5 (AT2R5 + Inh + CdCl2).*, p ≤ 0.05.