TABLE III.
Real-time PCR confirmation of differentially expressed apoptosis genesa
| Accession No. | Gene Symbol | Gene Name | p Value for Comparison of HIV-1-Infected Individuals vs Controls (Quantitative PCR) | Ratio of Signal between HIV-1-Infected Individuals and Controls |
|---|---|---|---|---|
| N98591 | IL6 | IL-6 | 0.0004 | 166.40 |
| W92812 | PPBP | Proplatelet basic protein (chemokine (C-X-C motif) ligand 7) | 0.0010 | 3.58 |
| AA425102 | CCL2 | Chemokine (C-C motif) ligand 2 | 0.0001 | 12.85 |
| NM_002984 | CCL4 | Chemokine (C-C motif) ligand 4 | 0.0050 | 5.99 |
| AA457705 | IER3 | Immediate early response 3 | 0.0071 | 3.06 |
| H77597 | MT1H | Metallothionein 1H | 0.0002 | 8.58 |
| H53340 | MT1G | Metallothionein 1G | 0.0008 | 5.09 |
| T49159 | PAI2 | Serine (or cysteine) proteinase inhibitor, clade B (OVA), member 2 | 0.0043 | 6.88 |
| W02699 | CD153 | CD30L | 0.0004 | 0.38 |
| AA167728 | SIVA | CD27-binding (Siva) protein | 0.0180 | 0.48 |
| AI952615 | p21 | CDKN1A/Waf1/Cip1/Sdi1 | 0.0220 | 1.54 |
Differentially expressed apoptosis genes validated by real-time PCR are shown. The accessions, gene symbols, gene names, p values, and fold ratios for apoptosis genes differentially expressed between HIV-1 and control monocytes (all but CCL4 and p21 were also identified as significant by microarray analysis) were confirmed for significance by real-time PCR analysis and are indicated.