Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2010 Apr 1.

Published in final edited form as: Mol Microbiol. 2009 Apr 21;72(4):844–858. doi: 10.1111/j.1365-2958.2009.06699.x

Fig 1 — Unstressed cells do not depend upon manganese. A. In standard medium, manganese import is too slight to activate MnSOD. Where indicated, media were unsupplemented or were supplemented with 50 μM manganese, and cell extracts were assayed before and after in vitro demetallation/reactivation with manganese. Each strain lacks sodB, to permit assay of MnSOD. The strains were AA138 (mntH⁺), AA141 (ΔmntH), and AA141/pAA01 (ΔmntH/pmntH). B. Manganese import is unnecessary for rapid growth. Wild-type MG1655 cultured without (open circles) or with (closed circles) 50 μM manganese supplement, and ΔmntH (AA99, plus sign) without manganese.

Fig 1 — Unstressed cells do not depend upon manganese. A. In standard medium, manganese import is too slight to activate MnSOD. Where indicated, media were unsupplemented or were supplemented with 50 μM manganese, and cell extracts were assayed before and after in vitro demetallation/reactivation with manganese. Each strain lacks sodB, to permit assay of MnSOD. The strains were AA138 (mntH⁺), AA141 (ΔmntH), and AA141/pAA01 (ΔmntH/pmntH). B. Manganese import is unnecessary for rapid growth. Wild-type MG1655 cultured without (open circles) or with (closed circles) 50 μM manganese supplement, and ΔmntH (AA99, plus sign) without manganese.