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. Author manuscript; available in PMC: 2010 Apr 1.

Published in final edited form as: Mol Microbiol. 2009 Apr 21;72(4):844–858. doi: 10.1111/j.1365-2958.2009.06699.x

Fig 6 — The primary role of intracellular manganese is not to degrade O₂^-. A. Metallation of MnSOD is not the primary purpose of manganese import. Cells were precultured in anaerobic defined medium (glucose/amino acids) and then aerated at time zero. LC106 (Hpx^-, squares), AA30 (Hpx^- ΔmntH, diamonds) and SMV30 (Hpx^- ΔsodA, X’s). B. Overproduction of MnSOD debilitates Hpx^- ΔmntH cells. Anaerobic cells, with or without the pDT1.5 sodA over-expression plasmid, were aerated at time zero in defined medium (glucose/amino acids) containing 100 μM MnCl₂. At the indicated time (arrow), manganese was removed. LC106 (Hpx^-, open squares), LC106/pDT1.5 (closed squares) and SMV42/pDT1.5 (Hpx^- ΔmntH, closed diamonds).

Fig 6 — The primary role of intracellular manganese is not to degrade O₂^-. A. Metallation of MnSOD is not the primary purpose of manganese import. Cells were precultured in anaerobic defined medium (glucose/amino acids) and then aerated at time zero. LC106 (Hpx^-, squares), AA30 (Hpx^- ΔmntH, diamonds) and SMV30 (Hpx^- ΔsodA, X’s). B. Overproduction of MnSOD debilitates Hpx^- ΔmntH cells. Anaerobic cells, with or without the pDT1.5 sodA over-expression plasmid, were aerated at time zero in defined medium (glucose/amino acids) containing 100 μM MnCl₂. At the indicated time (arrow), manganese was removed. LC106 (Hpx^-, open squares), LC106/pDT1.5 (closed squares) and SMV42/pDT1.5 (Hpx^- ΔmntH, closed diamonds).