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. Author manuscript; available in PMC: 2010 Apr 1.

Published in final edited form as: Mol Microbiol. 2009 Apr 21;72(4):844–858. doi: 10.1111/j.1365-2958.2009.06699.x

Fig 8 — Manganese is required to rescue Hpx^- cells from iron overload. A. Manganese supplementation rescues Hpx^- Δdps cells. Cells growing in anaerobic defined medium (glucose/amino acids), +/- 50 μM MnCl₂, were aerated at time zero. LC106 (Hpx^-, open squares), SP66 (Hpx^- Δdps, open diamonds), SP66 grown + MnCl₂ (closed diamonds). B. The growth defect of Hpx^- ΔmntH was suppressed by an iron-specific chelator. Cells (AA30) were grown in aerobic defined medium (glucose/amino acids) without MnCl₂ (open diamonds), with 1 μM MnCl₂ (dotted line, closed diamonds) or with 1 μM MnCl₂ plus 0.1 mM deferoxamine (closed diamonds).

Fig 8 — Manganese is required to rescue Hpx^- cells from iron overload. A. Manganese supplementation rescues Hpx^- Δdps cells. Cells growing in anaerobic defined medium (glucose/amino acids), +/- 50 μM MnCl₂, were aerated at time zero. LC106 (Hpx^-, open squares), SP66 (Hpx^- Δdps, open diamonds), SP66 grown + MnCl₂ (closed diamonds). B. The growth defect of Hpx^- ΔmntH was suppressed by an iron-specific chelator. Cells (AA30) were grown in aerobic defined medium (glucose/amino acids) without MnCl₂ (open diamonds), with 1 μM MnCl₂ (dotted line, closed diamonds) or with 1 μM MnCl₂ plus 0.1 mM deferoxamine (closed diamonds).