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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Mol Microbiol. 2009 Apr 21;72(4):844–858. doi: 10.1111/j.1365-2958.2009.06699.x

Fig 9.

Fig 9

Imported manganese suppresses oxidative protein carbonylation. Cells were grown in aerobic defined medium (glucose/amino acids) +/- 50 μM MnCl2, and proteins were harvested at an OD600 of ~ 0.2. Proteins were derivatized and western blotted as described in experimental procedures. The arrow indicates a non-oxidative carbonylation that was detected even when cells were grown and derivatized anaerobically. The strains used were MG1655 (wild-type), AA99 (ΔmntH), LC106 (Hpx-), AA30 (Hpx- ΔmntH) and SP66 (Hpx- Δdps).