FIGURE 5.

Absence of TRAIL does not rescue cells primed by a helper-dependent immunogen. Mice of the indicated genotypes were immunized with 10⁷ irradiated Act-mOVA splenocytes s.c. and 10 days later were infected (B) or not (A) with 4 × 10⁴ CFU LmOVA i.v. The OVA-specific response was analyzed 4 days later (day 14 after cellular immunization) by ICS in the spleen. FACS plots are gated on CD8⁺ T cells. Numbers indicate the frequency of cytokine-producing CD8⁺ T cells in each gate. C, The absolute number of IFN-γ-producing cells was quantified in the spleen. Frequencies were determined by subtracting the staining from the no peptide control. Naive WT mice infected with LmOVA 4 days before are shown as a control. Data represent the average of at least two mice per genotype ± SEM.