Figure 1.

Primary CD8⁺ T cell responses to HY coincide with the clearance of male cells. Female mice were injected with 2 × 10⁷ live male splenocytes and survival of donor cells and host CD8⁺ T cell responses were followed. At the indicated time points, splenocytes were stained directly ex vivo with H-2D^b/Smcy-specific tetramers or stimulated in vitro with Uty, Smcy, or no peptide and stained for levels of intracellular IFN-γ. Numbers indicate the percentage of IFN-γ⁺ cells in the CD8⁺ population. A, Response of host CD8⁺ T cells to Uty or Smcy peptides in the spleen. B, HY-specific CD8⁺ T cells measured by intracellular IFN-γ production as well as H-2D^b/Smcy-specific tetramer staining. Results are representative of two independent experiments. C, Total HY-specific CD8⁺ T cell responses were followed for 4 wk postimmunization with male cells. The values represent the combined response to the dominant epitopes Uty and Smcy measured by ICS. Error bars, SEM. Results are representative of six independent experiments. D, Survival of donor male or female UbC-GFP-transgenic cells in the spleen of host mice. Error bars, SEM (n = 3 for each time point).