Figure 4.

Direct Ag presentation by donor male cells is not necessary for the rapid Dby-specific T cell response, but host CD4⁺ T cell responses are necessary for rejection of male cells and development of CD8⁺ effector T cell responses. A, Female mice received 2 × 10⁷ live male MHC class II-deficient splenocytes and CD4⁺ and CD8⁺ T cell responses were monitored. Values represent the combined percentage of IFN-γ⁺ cells in the CD8⁺ T cell population responding to Uty and Smcy or the percentage of Dby-specific cells in the CD4⁺ T cell population. Results are representative of three separate experiments with two mice per time point. B, MHC class II-deficient female mice received 2 × 10⁷ B6 male splenocytes. Response of host CD8⁺ T cells to Uty or Smcy peptides in the spleen were analyzed at various time points. Numbers indicate the percentage of IFN-γ⁺ cells in the CD8⁺ population.