Figure 5.

MHC class II-restricted cytolytic activity following immunization with male cells is detectable 9 days postimmunization. A, Top panels, equal numbers of Ly5.1 CFSE^high Dby peptide-pulsed and CFSE^low control peptide-pulsed female splenocytes were injected i.v. into Ly5.2 naive or day 9 immune female mice. In vivo cytotoxicity was assessed in the lymph nodes 48 h after transfer. Representative histograms are gated on Ly5.1⁺MHC class II^high donor cells. Numbers at the top of each plot represent the percentage of CFSE^low or CFSE^high cells of the total Ly5.1⁺MHC class II^high donor cells recovered. A, Lower panels, equal numbers of Ly5.1 β2m^−/− CFSE^high male and CFSE^low female splenocytes were coinjected into Ly5.2 naive or day 9 immune NK-depleted female mice. In vivo cytotoxicity was assessed in the lymph nodes 48 h after transfer. Representative histograms are gated on Ly5.1⁺MHC class II^high donor cells. Numbers at the top of each plot represent the percentage of CFSE^low or CFSE^high cells of the total Ly5.1⁺MHC class II^high donor cells recovered. B, Cumulative data from three independent experiments of three mice per group. Error bars, SEM.