Figure 8.

Genetic determinants of break-induced isochromosome formation. (A) Percentage DSB-induced marker loss of strains carrying Ch¹⁶-RMGAH in wild-type (wt) (TH2130-3, TH2357) and cdc27-D1 (TH4460-1, TH4463-4) backgrounds at 30°C. The levels of non-homologous end joining/sister chromatid conversion (NHEJ/SCC), gene conversion (GC), minichromosome loss (Ch¹⁶ loss), and LOH are shown. s.e.m. values are indicated. (B) High-resolution PFGE analysis of parental wild-type (lane 1) and individual arg⁺G418^S his⁻ade⁻ (LOH) colonies from wild-type (lane 2) and cdc27-D1 background (lane 3–4) after DSB induction. (C) Percentage DSB-induced marker loss in lig4Δ (TH3569-70, TH3575-7), rad22Δ (TH4135-7, TH4138-9) and rad16Δ (TH4156-9, TH4161-2) backgrounds with outcome classification as above. s.e.m. values are indicated.