Table 1.
Compounds accumulated in the culture broths when mutants of P. putida U, specifically disrupted in a PhAc catabolic gene, were cultured in MM containing 4-OH-PhAc + PhAc or 4-OH-PhAc + PhH
| Additions to MM | Compound accumulated when gene disrupted
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| phaA | phaB | phaC | phaD | phaE | phaF | phaG | phaH | phaI | phaL | |
| 4-OH-PhAc (5 mM) + PhAc (5 mM) | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | 2-OH-PhAc |
| 4-OH-PhAc (5 mM) + PhH (5 mM) | PhAc + 2-OH-PhAc | PhAc + 2-OH-PhAc | PhAc | PhAc + muconic acid (traces) | None | PhAc | PhAc | PhAc | PhAc | 2-OH-PhAc |
Muconic acid is trans-trans-1,3-butadiene-1,4-dicarboxylic acid. Results similar to those when 4-OH-PhAc and PhH were added were obtained when P. putida U was cultured in MM + 4-OH-PhAc (5 mM) + PhO (5 mM).