Table 1.
Additions to MM | Compound accumulated when gene disrupted
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|
phaA | phaB | phaC | phaD | phaE | phaF | phaG | phaH | phaI | phaL | |
4-OH-PhAc (5 mM) + PhAc (5 mM) | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | PhAc | 2-OH-PhAc |
4-OH-PhAc (5 mM) + PhH (5 mM) | PhAc + 2-OH-PhAc | PhAc + 2-OH-PhAc | PhAc | PhAc + muconic acid (traces) | None | PhAc | PhAc | PhAc | PhAc | 2-OH-PhAc |
Muconic acid is trans-trans-1,3-butadiene-1,4-dicarboxylic acid. Results similar to those when 4-OH-PhAc and PhH were added were obtained when P. putida U was cultured in MM + 4-OH-PhAc (5 mM) + PhO (5 mM).