Figure 4.

Deletion of lag2 combined with the deletion of either dcn1 or rub1 inhibits the growth of W303-1A cells. (A) The indicated yeast cells were grown to log phase in YPD medium. Cells were harvested and resuspended in YPD medium to an OD₆₀₀ of 0.4; 3 μl of 10-fold serial dilutions was spotted onto YPD plates, incubated for 24 h at the indicated temperature, and photographed. (B) The indicated cells were plated onto the YPD plates, grown for 6 days at 30°C, and photographed. (C) The diameter of the colonies shown in (B) was measured. Data are expressed as a percentage of the size of W303-1A wild-type cells and are the means±s.d. from 60 independent colonies. (D) Overnight cultures of the indicated cells were inoculated into YPD medium at a density of an OD₆₀₀=0.05. Samples were taken every 1 h to measure the cell density.