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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: J Biol Chem. 2006 Jan 5;281(10):6768–6775. doi: 10.1074/jbc.M509687200

FIGURE 1. SDS-PAGE analysis and autophosphorylation assays of RegB.

FIGURE 1

A, solubilized and purified RegB separated by SDS-PAGE. Lane 1 contains the molecular weight mass followed by the wash fraction and the elution fraction after the addition of imidazole. B, autophosphorylation of purified monomer RegB using γ-32P-labeled ATP as a tracer. The aliquots were removed at 0.25, 0.5, 0.75, 1, 2, 4, 10, and 15 min after the addition of ATP, and the reaction was quenched with SDS-PAGE loading buffer before being separated by SDS-PAGE. The graph below the autoradiograph represents the arbitrary units of 32P incorporation derived from phosphorimaging data analysis.