Table 1.

Colocalizing fractions of NCX and CAV3 with RyR and of RyR with NCX and CAV3 (mean ± SD)

Protein A	% of protein A labeling colocalizing with RyR clusters	% of RyR cluster centroids (weighted by their relative intensities) colocalizing with protein A	Cells (n)	Animals (n)
NCX (t-tubular)∗	34.91 ± 5.99	41.73 ± 8.64	6	3
NCX (t-tubular)†	27.33 ± 2.22	55.17 ± 4.89	6	3
CAV3 (t-tubular)†	14.89 ± 2.59	13.99 ± 3.72	5	2
NCX (surface)†	26.62 ± 1.96	28.75 ± 2.20	5	2

^∗All puncta, weighted by their relative intensities, detected within a 150-nm distance of the nearest punctum of the second channel were considered colocalized.

^†First column shows the fraction of the total NCX/CAV3 labeling found within the RyR binary mask. The second column indicates the percentage of RyR labeling detected within the NCX/CAV3 binary mask.