Figure 5.

Nuclear EGFR expression contributes to double strand break repair. A. circular or HindIII-digested linear CMV-firefly luciferase plasmids were transfected into MCF7-EGFR expressing cells. TK-Renilla luciferase was co-transfected as tranfection efficiency control. Luciferase expression was measured after 24 hours of transfection. Repair activity was calculated by luciferase expression from linear plasmid divided with circular plasmid. B. MCF7-expressing cells were treated with 100 uM cisplatin 12 hours. Proteins wear harvested and subjected to Western blot with indicated antibodies. γH2A stands for S139 phosphorylated H2A.X and it is an indicator of DNA damage. γH2A expression after cisplatin treatment was calculated by densitometric analysis.