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. 2009 May 8;1(3):300–311.

Figure 1.

Figure 1

S. pneumoniae up-regulates MUC5AC mucin expression both in epithelial cells in vitro and middle ear and lung tissues of mouse in vivo. A, Cells were treated with S. pneumoniae strain D39, and total RNA was extracted from S. pneumoniae- or control-treated cells 5 hours after treatment. mRNA expression levels of MUC5AC mucin gene were measured by Q-PCR analysis. B, HM3 cells stably transfected with full size MUC5AC-Luc constructs were treated with S. pneumoniae strain D39 at various concentrations, and MUC5AC transcription was measured 5 hours after treatment by performing luciferase analysis. C, Cells were transfected with p3.7MUC5AC-Luc and treated with S. pneumoniae strain D39. MUC5AC transcription was measured 5 hours after S. pneumoniae or control treatment. D, C57BL/6 mice were inoculated with S. pneumoniae lysate into the middle ear via transtympanic membrane or into the lung via intratracheal inoculation. Bulla were isolated from S. pneumoniae- or control-inoculated mice 9 hours after inoculation, and lung tissues were dissected 6 hours after inoculation. Total RNA was extracted from the bulla and lung tissues, and mRNA expression levels of MUC5AC mucin gene were measured by Q-PCR analysis. The values presented in A – D are Mean ± SD (n = 3). *, p<0.05 compared with CON.