Figure 1.

Expression of α-smooth muscle actin in corneal myofibroblasts. The third passage corneal fibroblasts cultured on coverslips were washed with PBS, fixed with 4% paraformaldehyde, blocked in BSA/PBS, and sequentially incubated with monoclonal α-smooth muscle actin and FITC-conjugated secondary antibody. The nuclei were counterstained with 4'-6-diamidino-2-phenylindole(DAPI). Negative controls were performed using nonimmune serum instead of primary antibodies. A: Co-localization of α-SMA (green) and nuclei (blue) in myofibroblasts. B: Negative control.