Figure 2. Inflammatory and cytolytic effects of PLN on mouse alveolar macrophage MH-S cells.

MH-S cells were incubated with increasing doses of PLN for 6 hours with/without polymyxin B (10 µg/ml ) and TNF-α, MIP-2 and cell death were determined thereafter. Cell death was measured using MTT assay as described in Methods section. Data are mean±SEM (N = 5 per group). * P<0.05, † P<0.01 versus control.