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. 2009 Nov 24;4(11):e7972. doi: 10.1371/journal.pone.0007972

Figure 2. A comparison of the effects of different culture media used in ELISpot assays.

Figure 2

Spot numbers in un-stimulated and in M.tb PPD stimulated ex vivo ELISpot assays were determined following the use of different culture media (AIM-V serum-free medium; RPMI 1640 medium supplemented with heat inactivated human pooled AB serum (RPMI AB) or RPMI 1640 medium supplemented with autologous serum (RPMI AUT). Results are SFC per million PBMC in M.tb PPD-stimulated samples minus that measured in medium only samples. Box and whisker plots show the median, upper and lower quartiles and range of the response (n = 8). Statistical analysis is by paired t-tests.