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STAT activation by IL-TIF in HepG2 cells. HepG2 cells were stimulated for 15 min with hIL-TIF (1% 293-EBNA cell supernatant) or with a supernatant from mock-transfected cells. Gel shift assays were performed as described in Materials and Methods, with the Fcγ RI-derived GRR probe. STAT-containing complexes were supershifted with anti-STAT-1, -3, or -5 antibodies as indicated.
