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. 2000 Aug 22;97(18):10144–10149. doi: 10.1073/pnas.170291697

Figure 6.

Figure 6

In vivo SAA induction by IL-TIF. (A) Kinetics of induction of SAA by IL-TIF in vivo. Mice were injected i.p. with 50 μg of recombinant mIL-TIF. Mice were killed after various periods of time, and total RNA was extracted from the liver. Total RNA (10 μg) was fractionated by agarose gel electrophoresis and transferred to a nitrocellulose filter before hybridization with the SAA cDNA probe. Ethidium bromide-stained 18S and 28S ribosomal RNA show the equal loading of the gel. (B) Dose-dependent induction of SAA by IL-TIF. Mice were injected i.p. with various amounts of recombinant mIL-TIF and were killed 6 h after injection. Total liver RNA (10 μg) was fractionated by agarose gel electrophoresis and transferred to a nitrocellulose filter before hybridization with the SAA cDNA probe. Ethidium bromide-stained 18S and 28S ribosomal RNA show the equal loading of the gel.