Fig. 4.

Taxol induced cell death is not enhanced in partial Mps1 depleted immortalized fibroblasts. (A) VH10-TetRMps1 cells that were treated with and without dox for 4 days were immunoblotted for Mps1 and α-tubulin. Indicated amounts of untreated control sample were loaded to determine knockdown of Mps1 in dox treated sample. (B) Live cell analysis of the mitotic duration of indicated cell lines treated and measured as in Fig. 1B. (C) Quantification of colony formations as in Fig. 2. Average of three independent experiments is shown. (D) Quantification of time-lapse analysis (DIC) of chromosome segregation in anaphase. n = amount of cells filmed.