Fig. 1.

TRPM1 is expressed by ON-bipolar cells in the mouse retina. (A) In situ hybridization of vertical sections of mouse retina with antisense (Left) and sense control (Right) TRPM1 probes. A hybridization signal is detected in many cell somata in the INL, where bipolar cell nuclei and somata are located (black arrows). Occasional unlabeled cell somata are likely horizontal cells (white arrowhead). (B) Vertical sections from a wild-type (Top) and TRPM1^−/− (Bottom) retina were immunofluorescently labeled by antibodies directed against TRPM1 (green) and PKCα (red). Areas of colocalization appear yellow in the merged images (Right). onl, Outer nuclear layer; opl, outer plexiform layer; inl, inner nuclear layer; ipl, inner plexiform layer.