Figure 6.

Northern blot hybridization analyses of total RNA from the R. solani isolate Rhs 1A1 electrophoresed in a nondenaturing gel. Blots were hybridized with sense polarity (A) and complementary-sense polarity (B), ³²P-labeled ssRNA probes transcribed in vitro from a 3.4-kbp cDNA clone (M2–31) of M2 dsRNA. Positions of M2 dsRNA (3.6 kbp) and 3.6-kb ssRNA, which migrates to a position corresponding to 1.5 kbp (based on the 1-kbp DNA ladder) (15), are indicated on the right. Total RNA from Rhs 1AP did not hybridize with M2-specific RNA probes of either polarity (data not shown).