Spatial expression of Xenopus δ-catenin. (A) As viewed in
animal versus vegetal regions, whole-mount in situ RNA hybridization detects
δ-catenin mRNA signals in the ectoderm regions of blastula (subpanels
A-C) and gastrula (subpanel D) embryos. At neurulation (subpanel E), the
anterior and dorsal neural regions displayed the most apparent signals.
Embryos at tadpole stages (subpanel F) showed a distinctive staining pattern
in tissues of neural derivation such as brain, eye vesicle, ear vesicle,
branchial arches (higher magnification in subpanel G) and spinal cord as well
as somites (higher magnification in subpanel H). Subpanels I-L are
cross-section views of paraffin-fixed embryos from corresponding stages. Sense
probe hybridization was processed in parallel as negative controls (subpanels
M-O). (B) RT-PCR analyses detect δ-catenin transcripts in all adult
Xenopus tissues examined, with stronger expression in brain, nerve,
muscle and skin. (C) Immunoblotting using an N-terminus-directed antibody
detected three δ-catenin isoforms migrating at approximately 160, 130
and 100 kDa. The 130 and 100 kDa isoforms are ubiquitously present, whereas
the 160 kDa appears to be brain specific. An antibody directed against the
δ-catenin C-terminus reacts with the 160 kDa and 130 kDa isoforms in
brain.