Table 1. Percentage of dendritic cell subpopulations positive and negative for CPMV.
CD11chigh | CD11clow | CD11bhigh | CD11blow | CD80high | CD80low | CD86high | CD86low | |
Binding | ||||||||
CPMV(+) | 74.0 | 26.0 | 84.1 | 15.9 | 10.6 | 89.4 | 10.3 | 89.3 |
CPMV(−) | 53.7 | 46.3 | 53.0 | 47.0 | 10.8 | 89.1 | 21.7 | 78.3 |
Internalization | ||||||||
CPMV(+) | 88.5 | 11.5 | 86.0 | 13.9 | 8.4 | 91.5 | 5.4 | 94.5 |
CPMV(−) | 10.6 | 89.4 | 1.4 | 98.5 | 15.0 | 85.0 | 16.3 | 83.6 |
FACS analysis of immature dendritic cell subpopulations ability to bind and internalize CPMV-AF488. Subpopulations include high and low expression of CD11c, CD11b, CD80, and CD86. The majority of cells that bound and internalized CPMV expressed higher levels of CD11c and CD11b molecules, while having low expression of CD80 and CD86.