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. 2009 Nov 23;4(11):e7949. doi: 10.1371/journal.pone.0007949

Figure 3. MS/MS spectra studies of tryptic peptide reveal glutathionylation of Nm23-H1.

Figure 3

(A) Purified recombinant proteins, wild type Nm23-H1 and C109A mutant, were treated with and without 2.5 mM oxidized glutathione (GSSG), 5 mM reduced glutathione (GSH) at 37°C for 1 h. Each sample was separated on 12% SDS-PAGE under non-reducing condition and detected with coomassie blue staining. Only glutathionylation of wild Nm23-H1, not C109A mutant, was easily observed. (B) Tandem MS spectra of glutathionylated cellular and recombinant Nm23-H1 were compared. Both mass spectra profile was identical, only abundance of glutathionylated peptide is higher in recombinant protein.