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. 2009 Nov 23;4(11):e7949. doi: 10.1371/journal.pone.0007949

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Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Recombinant wild type Nm23-H1 was treated with 5.0 mM glutathione for 30 min at 37°C. Each sample was separated on 12% SDS-PAGE under non-reducing condition and detected with silver staining. (B) NDP kinase activity of each sample was measured as described in Experimental procedure. Glutathionylated fraction of sample lost their NDP kinase activity.