Figure 7. Impaired NDP kinase enzyme activity of oxidized Nm23-H1 is restored by of NADPH-TrxR-Trx system.

(A,B) Recombinant Nm23-H1 proteins (100 µM) were oxidized and reincubated with 200 µM NADPH, 80 µM rat TrxR1 and various concentration of Trx (0, 10 and 50 µM) same as in Figure 5. Enzymatic activities were measured on TLC plate (A) and quantitatively analyzed (B). (A) Same samples were separated on % SDS-PAGE under non-reducing condition.