Figure 9. Oxidative stress inhibits the metastasis suppression potential of Nm23-H1.

Invasion potential of MDA-MB-231 cells transiently transfected with pFlag-CMV-2, pFlag-Nm23-H1 or pFlag-Nm23-H1 C109A mutant were examined. Western analysis of cell lysates using indicated antibody were employed after separation on 12% SDS-PAGE. Cells overexpressing pFlag-CMV-2, pFlag-Nm23-H1, pFlag-Nm23-H1 C109A mutant or pFlag-Nm23-H1 H118F mutant were exposed to 0.5 mM H₂O₂ for 1 h (B) or none (A). And they were seeded on matrigel-pre-coated upper chamber (3×10⁴ cells/150 µL) and invasion potentials were measured.