Table 1. List of primary antibodies used and dilutions.
|
Primary antibody |
Host |
Dilution |
Source |
| Nestin |
Mouse |
1:400 |
BD Biosciences |
| Pax6 |
Rabbit |
1:1,000 |
Chemicon |
| β-tubulin III |
Mouse |
1:100 |
Chemicon |
| rhodopsin (4D2) |
Mouse |
1:100 |
Kind gift of R. Molday [32] |
| HuC/HuD |
Mouse |
1:200 |
Molecular Probes |
| recoverin |
Rabbit |
1:1,000 |
Kind gift of K. Koch [33] |
| Islet-1 |
Mouse |
1:500 |
Developmental Studies; Hybridoma Bank* |
| calbindin |
Rabbit |
1:1,000 |
SWANT |
| neurofilament-M |
Rabbit |
1:200 |
Chemicon |
| GFAP | Rabbit | 1:500 | Dako |
*The monoclonal antibody developed by T.M. Jessell was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA-52242.