Table 2. List of primers and cycling conditions for RT-PCR.
|
Gene |
Primer sequence (5'-3') |
Product size (bp) |
| Nestin1 |
F: GGCTTCTCTCAGCATCTTGG |
|
| R: AAGGCTGGCATAGGTGTGTC |
150 |
|
| β-tubulin III2 |
F: CAGAGCAAGAACAGCAGCTACTT |
|
| R: GTGAACTCCATCTCGTCCATGCCCTC |
250 |
|
| GFAP1 |
F: TTGACCTGCGACGTGGAGTC |
|
| R: AGGTGGCGATCTCGATGTCC |
225 |
|
| β-actin2 |
F: CTTCCCCTCCATCGTGGG |
|
| R: GTGGTACGGCCAGAGGCG | 355 |
1Amplification conditions were 1 min/94 °C, 1.5 min/57 °C, 1.5 min/72 °C for 32 cycles. 2Amplification conditions were 1 min/94 °C, 1.5 min/56°C, 1.5 min/72 °C for 30 cycles.