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. Author manuscript; available in PMC: 2009 Nov 12.

Published in final edited form as: J Cell Biochem. 2009 Mar 1;106(4):563–569. doi: 10.1002/jcb.22021

A. Representative micrographs of TRAP positive (upper panels), vitronectin positive (middle panels) multinuclear cells and bone resorption (lower panel). 0.5×10⁶ CD11b+ cells were all cultured with M-CSF (25 ng/ml) and neutralizing antibodies for IL-6 and IL-8 and RANKFc with 50% conditioned media from PC-3 cells. Graphical representation of number of osteoclast-like multinuclear TRAP positive cells (B) and bone resorption area (C).

A. Representative micrographs of TRAP positive (upper panels), vitronectin positive (middle panels) multinuclear cells and bone resorption (lower panel). 0.5×10⁶ CD11b+ cells were all cultured with M-CSF (25 ng/ml) and neutralizing antibodies for IL-6 and IL-8 and RANKFc with 50% conditioned media from PC-3 cells. Graphical representation of number of osteoclast-like multinuclear TRAP positive cells (B) and bone resorption area (C).