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. Author manuscript; available in PMC: 2009 Nov 12.
Published in final edited form as: Oncogene. 2008 May 26;27(37):5057–5068. doi: 10.1038/onc.2008.143

Figure 3.

Figure 3

Elevation of p66Shc prior to reactive oxygen species (ROS) production and cell growth induction in dihydrotestosterone (DHT)-treated LNCaP cells. LNCaP cells were plated and steroid starved for 48 h. Cells were then treated with 10 nM DHT and control cells were treated with equal volume of ethanol. Cells were harvested at specific time periods and were analysed for (a) Shc isoforms, Cyclin D1 and secreted prostate-specific antigen (sPSA) protein levels by immunoblotting, (b) ROS production using DCF-DA analysis (*P<0.05), (c) cell cycle analysis (*P<0.01) by flow cytometry and (d) cell growth analysis (*P<0.01) by cell counting.